【英文标准名称】:StandardGuideforConductingRenewalPhytotoxicityTestsWithFreshwaterEmergentMacrophytes
【原文标准名称】:淡水中露出水面的大型植物的植物毒素重新检测的标准指南
【标准号】:ASTME1841-2004
【标准状态】:现行
【国别】:
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.01
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:重新;水;微生物学;毒性;淡水
【英文主题词】:chlorophyll;emergentmacrophyte;phytotoxicitytest;surrogatespecies
【摘要】:Increasedemphasisisbeingplacedonprotectingwetlands(6)andseveralagenciesincludingU.S.EnvironmentalProtectionAgencyandEnvironmentCanadaarebeginningtorequire,fortheregistrationofpesticides,dataregardingtoxicityoftestmaterialstorootedaquaticvascularplants(7,8,9).Muchresearchisbeingconductedwithvascularplants,bothterrestrialandaquatic(10),however,protocolsforphytotoxicitytestingwithfreshwateremergentmacrophytesstillarenotwelldefined.Thisguideisdesignedtoassesspotentialdetrimentaleffectsofwatersolublechemicalsubstancesonselectedsurrogatespeciesoffreshwateremergentmacrophytes.Thisguidefocusesondiminishmentofchlorophyllcontentinleavesasthemeasurableendpoint,however,notallchemicalsaffectchlorophyllproduction.DryweightcanbeusedastheendpointforO.sativa,however,exposuretimesmayneedtobeextendedtodetecttreatmentdifferences.Dryweightisnotarecommendedendpointforanyofthetestspeciesstartedasrhizomesortubers.Otherendpoints,suchasperoxidaseactivity(11)orchlorophyllfluorescence(12)couldpossiblybeused.Thisguidecouldbeusedtoprovideearlyindicationofpotentialproblems,identifyhazardoussubstancesbeforecontaminationofwetlandsoccurs,andestablishx201C;marginsofsafetyx201D;forspecificchemicalswithinwetlands(seeGuideE1023).Thisguideisnotdesignedtoreplacefieldassessmentsorotheraquatictestingprocedures.Itisdesignedtocomplimentsuchtesting,sothatamorecompleteassessmentispossible.1.1Thistestguideisdesignedtogivegeneralguidanceforassessingthepotentialphytotoxicityofwatersolubletestmaterialtofreshwateremergentmacrophytes.1.2Thisrenewaltestcontinuouslyexposesselectedplantspecies,growinginsediment,tovariousconcentrationsoftestmaterial,dissolvedinanutrientsolution.1.3ThistestguideisbasedontheToxicSubstancesControlAct(TSCA)guidelinesforconductingtoxicitytestswithterrestrialplants(1)andisapplicabletomostwatersolublechemicals,eitherindividuallyorinformulations,commercialproducts,orknownmixtures(seeGuidesE1193andE1598).Withslightmodificationstheprocedurealsomightbeusedforeffluents(seeGuideE1192).1.4ResultsfromthistoxicitytestcanbeusedtoreportanIC50orNOEC(seeSection3)basedontheconcentrationofchlorophyllextractedfromtheplants(seeGuidesD3731andE1218).Insomesituations,itmightbenecessarytoonlytestatoneconcentrationtodeterminewhetherornotthatspecificconcentrationistoxictotheplants.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.SpecifichazardstatementsaregiveninSection7.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20;13_060_70
【页数】:10P.;A4
【正文语种】: